Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.*

Posted by on in 2014
  • Font size: Larger Smaller
  • Hits: 1655
  • Print

Van Deventer JA1Wittrup KD. 2014. Methods Mol Biol.1131:151-81. doi: 10.1007/978-1-62703-992-5_10. 1. Department of Chemical Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

Abstract

Antibodies play key roles as reagents, diagnostics, and therapeutics in numerous biological and biomedical research settings. Although many antibodies are commercially available, oftentimes, specific applications require the development of antibodies with customized properties. Yeast surface display is a robust, versatile, and quantitative method for generating these antibodies and is accessible to single-investigator laboratories. This protocol details the key aspects of yeast surface display library construction and screening.

*Note The Chicken anti-CMYC used in this publication was produced by Gallus Immunotech Inc.

Buy it here:

PMID: 24515465[PubMed - in process]

Last modified on