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Electrical detection of dengue biomarker using egg yolk immunoglobulin as the biological recognition element.

Posted by on in 2015
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Figueiredo A1Vieira NC1Dos Santos JF1Janegitz BC1Aoki SM2Junior PP2Lovato RL2Nogueira ML3Zucolotto V1Guimarães FE1  Sci Rep. 2015 5:7865. doi: 10.1038/srep07865.

  • 1Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
  • 2DNAPTA Biotecnologia, 15035-470, São José do Rio Preto - SP, Brasil.
  • 3Departamento de Doenças Infecciosas e Parasitárias, FAMERP, 15090-000, São José do Rio Preto - SP, Brasil.

Abstract

Nonstructural protein 1 (NS1) is secreted by dengue virus in the first days of infection and acts as an excellent dengue biomarker. Here, the direct electrical detection of NS1 from dengue type 2 virus has been achieved by the measurement of variations in open circuit potential (OCP) between a reference electrode and a disposable Au electrode containing immobilized anti-NS1 antibodies acting as immunosensor. Egg yolk immunoglobulin (IgY) was utilized for the first time as the biological recognition element alternatively to conventional mammalian antibodies in the detection of dengue virus NS1 protein. NS1 protein was detected in standard samples in a 0.1 to 10 µg.mL(-1) concentration range with (3.2 ± 0.3) mV/µg.mL(-1) of sensitivity and 0.09 µg.mL(-1) of detection limit. Therefore, the proposed system can be extended to detect NS1 in real samples and provide an early diagnosis of dengue.

PMID:
 
25597820
 
[PubMed - in process]
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