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Effects of specific egg yolk immunoglobulin on pan-drug-resistant Acinetobacter baumannii.

Posted by on in 2017
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Shi H1Zhu J2Zou B3Shi L3Du L3Long Y3Wang H4Xu H4Zhen Y5Sun L6Biomed Pharmacother. 95:1734-1742. doi: 10.1016/j.biopha.2017.09.112. Epub 2017 Oct 6.

1
Affiliated Dalian Friendship Hospital of Dalian Medical University, Dalian, 116001, China.
2
The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116021, China.
3
College of Pharmacy, Dalian Medical University, Dalian, 116044, China.
4
College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, China.
5
College of Pharmacy, Dalian Medical University, Dalian, 116044, China. Electronic address: zhenyhwaner@aliyun.com.
6
Affiliated Dalian Friendship Hospital of Dalian Medical University, Dalian, 116001, China. Electronic address: sunlidanyyhx@163.com.

Abstract

With the growing emergence of pan-drug-resistant Acinetobacter baumannii (PDR-Ab) strains in clinical, new strategies for the treatment of PDR-Ab infections are urgently needed. Egg yolk immunoglobulin (IgY) as a convenient and inexpensive antibody has been widely applied to the therapy of infectious diseases. The aim of this study was to produce IgY specific to PDR-Ab and investigate its antibacterial effects in vitro and in vivo. IgYs specific to two PDR-Ab strains were produced by immunizing hens with formaldehyde inactivated PDR-Ab cells and isolated from yolks with a purity of 90% by water dilution, salt precipitations and ultrafiltration. IgYs showed high titers when subjected to an ELISA and inhibited the growth of PDR-Ab in a dose-dependent manner in liquid medium. Scanning electron microscopy assay showed structural modification and aggregation of PDR-Ab treated with specific IgYs. Freshly cultured PDR-Ab cells were nasally inhaled in BALB/c mice to induce acute pneumonia. The infected mice were intraperitoneally injected with specific IgYs using cefoperazone/sulbactam and dexamethasone as positive controls. The IgYs specific to PDR-Ab lowered the mortality of mice with PDR-Ab-induced acute pneumonia, decreased the level of TNF-α and IL-1β in serum and reduced inflammation in lung tissue. Specific IgY has the potential to be used as a new therapeutic approach for the treatment of A. baumannii-induced infections.

KEYWORDS:

A. baumannii; Acute pneumonia; Egg yolk immunoglobulin; Pan-drug-resistance

PMID:
 
28962078
 
DOI:
 
10.1016/j.biopha.2017.09.112
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