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Differences in IgY gut absorption in gastric rainbow trout (Oncorhynchus mykiss) and agastric common carp (Cyprinus carpio) assessed in vivo and in vitro.

Posted by on in 2015
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  • Winkelbach A1Günzel D2Schulz C3Wuertz S4. 2015. Comp Biochem Physiol C Toxicol Pharmacol. 167:58-64. doi: 10.1016/j.cbpc.2014.09.001. Epub 2014 Sep 16.
  • 1Department of Aquaculture and Ecophysiology, Leibniz Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany; Gesellschaft für Marine Aquakultur, Hafentörn 3, 25761 Büsum, Germany; Institute of Animal Breeding and Husbandry, Christian-Albrechts-Universität zu Kiel, Olshausenstraße 40, 24098 Kiel, Germany. Electronic address: winkelbach@gma-buesum.de.
  • 2Institute of Clinical Physiology, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany.
  • 3Gesellschaft für Marine Aquakultur, Hafentörn 3, 25761 Büsum, Germany; Institute of Animal Breeding and Husbandry, Christian-Albrechts-Universität zu Kiel, Olshausenstraße 40, 24098 Kiel, Germany.
  • 4Department of Aquaculture and Ecophysiology, Leibniz Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany.

Abstract

Oral IgY antibodies offer promising potential for passive immunization strategies. To evaluate barriers for successful IgY plasma recovery after oral application in vivo, gastric rainbow trout and agastric common carp were comparatively assessed. A positive control that received a low dose of unspecific IgY antibodies by intraperitoneal injection (0.0076mgIgYgBW(-1)d(-1); BW=body mass) was compared with an oral administration of 75 and 150 fold in rainbow trout (corresponding to 0.57 and 1.14mggBW(-1)) and in carp (0.57mggBW(-1)). Dietary antibodies were delivered with the antacid sodium bicarbonate and three different putative uptake enhancers (Tween 20, vitamin E TPGS, sodium deoxycholate). IgY concentrations in the plasma were determined 1d (rainbow trout) or 5d after last feeding (both species). Irrespective of the enhancer used, ELISA revealed IgYabsorption after feeding in carp, whereas IgY concentration in rainbow trout remained below the detection threshold. Intraperitoneal injections revealed IgY in plasma of both species. In vitro Ussing chamber experiments with posterior intestine tissue of carp and trout were carried out to determine whether species-specific differences in IgY translocation were due to acidic stomach passage or species-specific differences in transepithelial IgY passage. Significantly higher IgY translocation was measured in carp at high application dosage compared to all other groups, indicating that species-specific differences in IgY uptake after oral administration are not only related to peptic IgY degradation in the stomach, but also likely a result of differences in IgY transcytosis in the posterior intestine.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Carp; Enhancer; IgY; Oral; Passive immunization; Transcytosis; Trout; Ussing chamber

PMID:
 
25224559
 
[PubMed - in process]
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