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Development of IgY antibodies against anti-snake toxins endowed with highly lethal neutralizing activity.

Posted by on in 2017
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da Rocha DG1Fernandez JH2de Almeida CMC3da Silva CL1Magnoli FC4da Silva OÉ5da Silva WD6. 2017. Eur J Pharm Sci.106:404-412. doi: 10.1016/j.ejps.2017.05.069. Epub 2017 Jun 5.

Abstract

Snakebite envenoming is a major neglected disease related to poverty in developing countries. Treatment involves the administration of a specific antivenom serum and auxiliary therapies, if necessary. The improvement of antibodies is of great importance for the technological advancement of antivenom therapy and to reduce the morbidity and mortality associated with this medical burden. In the present study, adult hens were immunized nine times with 20μg of B. arietans or C. d. terrificus venoms at three-week intervals between immunizations. Developing antibodies presented increasing avidity and affinity to antigenic toxin epitopes along immunization, attaining a plateau after the seventh immunization. Pooled egg yolk-purified IgY antivenom antibodies, subjected to in vitro-in vivo lethality assay using Swiss adult mice, exhibited potent venom lethal neutralizing activity. Taken together, chickens under the described immunization schedule were considered alternative candidates for antivenom production. Lower maintenance costs, a simple antibody manufacturing process and immunization suffering restrictions are additional advantages.

KEYWORDS:

Affinity; Ammonium sulfate (PubChem CID: 24890861); Antivenoms; Avidity; DAB (PubChem CID: 24893950); ELISA; OPD (PubChem CID: 24898741); PEG 6000 (PubChem CID: 24887720); Titration; Tween 20 (PubChem CID: 443314); Urea (PubChem CID: 24900656)

PMID:
 
28595875
 
DOI:
 
10.1016/j.ejps.2017.05.069
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