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Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.*

Posted by on in 2017
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Bilverstone TW1Kinsmore NL2Minton NP3Kuehne SA2. 2017. Anaerobe. 44:51-54. doi: 10.1016/j.anaerobe.2017.01.009. Epub 2017 Jan 17.

1
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
2
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK.
3
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK. Electronic address: nigel.minton@nottingham.ac.uk.

Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

KEYWORDS:

Binary toxin; C. difficile; CdtR; PaLoc; Virulence

PMID:
 
28108389
 
PMCID:
 
PMC5408908
 
DOI:
 
10.1016/j.anaerobe.2017.01.009
[Indexed for MEDLINE] 
Free PMC Article
 

*Note: The HRP-labeled Chicken anti-Clostridium difficile Binary Toxin Subunit A used in this publication was manufactured by Gallus Immunotech Inc. 

 
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