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Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA.

Posted by on in 2017
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Li C1Zhang Y1Eremin SA2Yakup O3Yao G3Zhang X4. 2017. Food Chem.227:48-54. doi: 10.1016/j.foodchem.2017.01.058. Epub 2017 Jan 16.

1College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China.2Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia; Federal Research Center «Fundamentals of Biotechnology» of the Russian Academy of Sciences, 119071 Moscow, Russia.3College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.4College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China. Electronic address: zhang.xy@nwsuaf.edu.cn.

Abstract

Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50 of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC50 of FPIA and ic-ELISA based anti-Kana IgY were 7.97±0.9μg/mL and 0.15±0.01μg/mL. The limits of detection (LOD, IC10) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007μg/mL, respectively. The LOD for ic-ELISA were both 0.001μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.

KEYWORDS:

Fluorescence polarization immunoassay (FPIA); Gentamicin; Immunoglobulin Y (IgY); Indirect-competitive ELISA (ic-ELISA); Kanamycin

PMID:
 
28274457
 
DOI:
 
10.1016/j.foodchem.2017.01.058
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