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Comparison of colorimetric and chemiluminescent enzyme-linked immunosorbent assay for the detection of endosulfan in food samples.

Posted by on in 2015
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Deepak TS1Rashmi SManonmani HKLuminescence. 2015 Mar 24. doi: 10.1002/bio.2892. [Epub ahead of print]

1Department of Fermentation Technology and Bioengineering, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India.

Abstract

Pesticides have become part of food protection since their inception. Endosulfan, an organochlorine insecticide, has been used against insect pests such as whiteflies, aphids, red spiders and mites. Methods of immunochemical assays have been devised for the determination and analysis of pesticides and commonly used for the analysis of contaminants in food, water, soil and body fluids. Chicken IgY antibodies raised against endosulfan haptens were used for the detection of endosulfan. We have compared colorimetric (CO) and chemiluminescence (CL) enzyme-linked immunosorbent assay (ELISA) techniques for the detection of endosulfan isomers in a food matrix. CL ELISA assay was found to be more sensitive than CO assay. The mean recovery was 81.2-95.6% for α- and β-endosulfan-spiked food samples with 2.8-4.6% relative standard deviation. The detection of the endosulfan isomers was linear in the range 100 µg/mL-5 fg/mL, with a limit of detection at 100 µg/mL and 5 fg/mL for the CL ELISA method and 100 µg/mL and 1 ng/mL for the CO ELISA method respectively. These methods can be used for the rapid and reliable detection of organochlorine pesticide endosulfan. Copyright © 2015 John Wiley & Sons, Ltd.

Copyright © 2015 John Wiley & Sons, Ltd.

KEYWORDS:

ELISA; Endosulfan; IgY antibodies; chemiluminescence; colorimetric

PMID:
 
25808457
 
[PubMed - as supplied by publisher]
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