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Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis.

Posted by on in 2016
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Teimoori S1Arimatsu Y2Laha T3Kaewkes S3Sereerak P4Sripa M2Tangkawattana S4Brindley PJ5Sripa B62016. Parasitol Int.  pii: S1383-5769(16)30093-9. doi: 10.1016/j.parint.2015.10.011. [Epub ahead of print]

1Centre of Research Excellence for Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Bangkok 10700, Thailand; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
2WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
3Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
4Department of Pathobiology, Faculty of Veterinary, Medicine, Khon Kaen University, Khon Kaen, Thailand.
5Department of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Diseases of Poverty, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
6Centre of Research Excellence for Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Bangkok 10700, Thailand. Electronic address: banchob@kku.ac.th.


Abstract

Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. Therefore sensitive and specific diagnostic test is needed. In this study, a coproantigen sandwich ELISA using recombinant O. viverrini cathepsin F (rOv-CF) was developed. This sandwich ELISA employing chicken IgY raised against rOv-CF in combination with rabbit IgG antibody to the somatic O. viverrini antigens showed a lower detection limit (LLD) of 70ng native O. viverrini somatic antigens by spiking the parasite antigens into control feces. When applied to the diagnosis, the IgY-based sandwich ELISA exhibited sensitivity and specificity of 93.3% and 76.7%, respectively, in an investigation of 90 human cases positive or negative for opisthorchiasis. The positive predictive value (PPV) and negative predictive value (NPV) for this coproantigen detection were 66.7% and 95.2%, respectively. This IgY-based sandwich ELISA using parasite cathepsin F detection shows a promising immunodiagnostic alternative for human opisthorchiasis in endemic regions.

KEYWORDS:

Cathepsin F; Chicken IgY; Coproantigen; Immunodiagnosis; Opisthorchis viverrini; Sandwich ELISA

PMID:
 
27140305
 
PMCID:
 
PMC5086311
 [Available on 2017-10-29]
 
DOI:
 
10.1016/j.parint.2015.10.011
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