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Chicken egg yolk antibodies (IgY) modulate the intestinal mucosal immune response in a mouse model of Salmonella typhimurium infection.

Posted by on in 2016
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Li X1Yao Y2Wang X2Zhen Y3Thacker PA4Wang L1Shi M5Zhao J6Zong Y2Wang N2Xu Y72016. Int Immunopharmacol. 36:305-14. doi: 10.1016/j.intimp.2016.04.036. Epub 2016 May 21.

1
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Ministry of Education Center for Food Safety of Animal Origin, Dalian 116620, China.
2
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
3
Department of Pharmacy, Dalian Medical University, Dalian 116044, China.
4
Department of Animal and Poultry Science, University of Saskatchewan, Saskatchewan S7N5A8, Canada.
5
College of Medical, Dalian University, Dalian 116622, China.
6
College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China.
7
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Ministry of Education Center for Food Safety of Animal Origin, Dalian 116620, China. Electronic address: xiaoyuli@dlut.edu.cn.

 

Abstract

This study determined the effects of chicken egg yolk antibodies (IgY) on immune responses in the intestinal mucosal of mice infected with Salmonella typhimurium. Sixty, 28-day-old mice were divided into 4 groups and treated with streptomycin or sterile water for 2days followed by 1day without treatment. The control group was unchallenged whereas the mice in the other three groups were treated twice with 10(9)CFUmL(-1)S. typhimurium. For the next 3days, control mice continued to receive no treatment whereas the mice in the remaining three groups were orally administered with 20mgmL(-1) of specific IgY, 20mgmL(-1) of nonspecific IgY or PBS. S. typhimurium activated gut-associated lymphoid tissue, increasing the release of IFN-γ and TNF-α in the mucosa and increased the number of activated T-lymphocytes and cytotoxic T-γδ. Specific IgY attenuated the increase in IFN-γ and TNF-α and the decrease in IL-10. S. typhimurium induced mobilization of CD8(+) and CD8(+) TCRγδ T cells in the epithelium and CD4(+) and CD8(+) T cells in the lamina propria reflecting an inflammatory process that was attenuated by IgY. These results suggest that specific IgY modulates intestinal mucosal immune responses during a S. typhimurium infection.

KEYWORDS:

Egg yolk antibodies; Gut-associated lymphoid tissue; IgY; Intestinal inflammation; Mucosal immune response; Salmonella typhimurium

PMID:
 
27214338
 
DOI:
 
10.1016/j.intimp.2016.04.036
[Indexed for MEDLINE]
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