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Biological and Immunogenicity Property of IgY Anti S. mutans ComD.

Posted by on in 2016
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Bachtiar EW1Bachtiar BM1Soejoedono RD2Wibawan IW2Afdhal A1.2016. Open Dent J. 10:308-14. doi: 10.2174/1874210601610010308. eCollection 2016.

1Department of of Oral Biology, Faculty of Dentistry, Oral Sciences Research Center, Universitas Indonesia, Jakarta, Indonesia.
2Faculty of Veterinary Bogor, Institute of Agriculture, Bogor, Indonesia.

 

Abstract

OBJECTIVE:

This study aims to elucidate the effect of IgY anti ComD on the biological properties of Streptococcus mutans. (S. mutans) ComD is an interspecies quorum-sensing signaling receptor that plays an important role in biofilm formation by S. mutans.

MATERIALS AND METHODOLOGY:

Egg yolk IgY was produced by the immunization of chickens with a DNA vaccine containing the ComD DNA coding region. We evaluated the effect of the antibody on biofilm formation by S. mutans isolated from subjects with or without dental caries. We also assessed the immunoreactivity of the antibody against all isolates, and analyzed the protein profile of S. mutans by SDS-PAGE.

RESULTS:

The ComD antibody was successfully induced in the hens' eggs. It inhibited biofilm formation by all S. mutans isolates. In addition, the expression of some protein bands was affected after exposure to the antibody.

CONCLUSION:

IgY anti-S. mutans ComD reduces biofilm formation by this bacterium and alters the protein profile of S. mutans.

KEYWORDS:

Biofilm; Caries; Streptococcus mutans; Vaccine

PMID:
 
27386013
 
PMCID:
 
PMC4911422
 
DOI:
 
10.2174/1874210601610010308
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