Wei-Xu H1, Wen-Yun Z2, Xi-Ling Z2, Zhu W3, Li-Hua W2, Xiao-Mu W2, Hui-Ping W2, Wen-Ding W2, Dan H2, Qin X4, Guo-Zhu H2. 2016. Mediators Inflamm. 2016:3128182. doi: 10.1155/2016/3128182. Epub 2016 Mar 7.
1Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Shanghai 201315, China.
2Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang, Jiangxi 330006, China.
3Department of Hematology, Jiangxi Academy of Medical Science, Nanchang, Jiangxi 330006, China.
4State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, China.
This study aims to determine whether the combined blockade of IL-1β and TNF-α can alleviate the pathological allergic inflammatory reaction in the nasal mucosa and lung tissues in allergic rhinitis (AR) guinea pigs. Healthy guinea pigs treated with saline were used as the healthy controls. The AR guinea pigs were randomly divided into (1) the AR model group treated with intranasal saline; (2) the 0.1% nonspecific IgY treatment group; (3) the 0.1% anti-TNF-α IgY treatment group; (4) the 0.1% anti-IL-1β IgY treatment group; (5) the 0.1% combined anti-IL-1β and TNF-α IgY treatment group; and (6) the fluticasone propionate treatment group. The inflammatory cells were evaluated using Wright's staining. Histopathology was examined using hematoxylin-eosin staining. The results showed that the number of eosinophils was significantly decreased in the peripheral blood, nasal lavage fluid, and bronchoalveolar lavage fluid (P < 0.05), and eosinophil, neutrophil, and lymphocyte infiltration and edema were significantly reduced or absent in the nasal mucosa and lung tissues (P < 0.05) in the combined 0.1% anti-IL-1β- and TNF-α IgY-treated guinea pigs. The data suggest that topical blockade of IL-1β and TNF-α could reduce pathological allergic inflammation in the nasal mucosa and lung tissues in AR guinea pigs.