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A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.

Posted by on in 2017
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Song D1Qu X1Liu Y1Li L1Yin D2Li J1Xu K1Xie R3Zhai Y1Zhang H1Bao H1Zhao C1Wang J1Song X4Song W5Nanoscale Res Lett. 2017 Dec;12(1):179. doi: 10.1186/s11671-017-1941-z. Epub 2017 Mar 9.
Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
School of Public Health, Xuzhou Medical University, 221000, Xuzhou, China.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, 130000, Changchun, China.
Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
China-Japan Union Hospital, Jilin University, 130000, Changchun, China.


Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N'-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the Brucella and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 103 CFU/mL and ranges from 10 to 105 CFU/mL (R 2 = 0.9983), and it can be well used in real samples.


Fluorescence; IgG; IgY; Immunomagnetic beads; Quantum dots

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