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A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.

Posted by on in 2017
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Song D1Qu X1Liu Y1Li L1Yin D2Li J1Xu K1Xie R3Zhai Y1Zhang H1Bao H1Zhao C1Wang J1Song X4Song W5Nanoscale Res Lett. 2017 Dec;12(1):179. doi: 10.1186/s11671-017-1941-z. Epub 2017 Mar 9.
1
Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
2
School of Public Health, Xuzhou Medical University, 221000, Xuzhou, China.
3
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, 130000, Changchun, China.
4
Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China. songxiuling@jlu.edu.cn.
5
China-Japan Union Hospital, Jilin University, 130000, Changchun, China. songwz@jlu.edu.cn.

Abstract

Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N'-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the Brucella and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 103 CFU/mL and ranges from 10 to 105 CFU/mL (R 2 = 0.9983), and it can be well used in real samples.

KEYWORDS:

Fluorescence; IgG; IgY; Immunomagnetic beads; Quantum dots

PMID:
 
28282974
 
DOI:
 
10.1186/s11671-017-1941-z
 
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