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A comprehensive evaluation of an ELISA for the diagnosis of the two most common ascarids in chickens using plasma or egg yolks.

Posted by on in 2017
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Daş G1Hennies M2Sohnrey B3Rahimian S3Wongrak K4Stehr M5Gauly M6Parasit Vectors. 2017 Apr 18;10(1):187. doi: 10.1186/s13071-017-2121-9.

1
Institute of Nutritional Physiology 'Oskar Kellner', Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. gdas@fbn-dummerstorf.de.
2
TECOdevelopment GmbH, Marie-Curie-Str. 1, 53359, Rheinbach, Germany.
3
Department of Animal Sciences, University of Göttingen, Albrecht-Thaer-Weg 3, 37075, Göttingen, Germany.
4
Faculty of Agriculture and Life Science, Chandrakasem Rajabhat University, 39/1 Ratchadaphisek Road, Chatuchak, 10900, Bangkok, Thailand.
5
Institute of Nutritional Physiology 'Oskar Kellner', Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
6
Free University of Bozen - Bolzano, Faculty of Science and Technology, Universitätsplatz 5, 39100, Bolzano, Italy.

Abstract

BACKGROUND:

Classical faecal egg counts (FEC) provide less reliable diagnostic information for nematode infections in chickens. We developed an ELISA based on Ascaridia galli antigens and tested two hypotheses, as follows: (i) IgY antibodies developed against A. galli will also be useful to identify Heterakis gallinarum infections, and (ii) circulating antibodies stored in egg yolks are as good as plasma samples, so a non-invasive diagnosis is possible. The aim of this study, therefore, was to compare the diagnostic accuracy of the ELISA system with FEC, using both plasma and egg yolks from experimentally infected hens. In addition, naturally infected animals were evaluated to validate the assay.

RESULTS:

The assay quantified large differences (P < 0.001) in plasma or in egg-yolk IgY concentrations between infected and uninfected animals in two experiments, each performed with either of the nematode species. The assay performed with high accuracy as quantified with the area under the ROC curve (AUC) values of > 0.90 for both nematodes using either plasma or egg yolks. Sensitivity of the assay was 94 and 93% with plasma and egg yolk samples, respectively, whereas FEC yielded in a sensitivity of 84% in A. galli experiment. Total test accuracy of the assay with plasma samples (AUC = 0.99) tended to be higher (P = 0.0630) than FEC (AUC = 0.92) for A. galli, while the assay with either sample matrix performed similar to FEC (AUC ≥ 0.91) for H. gallinarum. Among the three tests, the FECs correlated better with A. galli burden than the ELISA. Although 90% of naturally infected hens were correctly identified by the ELISA, 45% of the infected hens tested negative with FEC, indicating the validity of the higher test accuracy of the ELISA.

CONCLUSIONS:

Antigens of A. galli can be used successfully to identify H. gallinarum-infected animals, indicating that chickens develop cross-reactive antibodies against the two closely related species. Egg yolks are as informative as plasma samples, so that animal welfare-friendly sampling is possible. Although the assay with plasma samples reveals qualitative information of higher quality than FECs on the infection status of naturally infected birds, the latter is still a better tool to assess the intensity of A. galli but not of H. gallinarum infections.

KEYWORDS:

Helminth; Nematode; Non-invasive diagnosis; Poultry; ROC analysis; Test accuracy

PMID:
 
28420423
 
PMCID:
 
PMC5395908
 
DOI:
 
10.1186/s13071-017-2121-9
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