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Differential Rates of Local and Global Homogenization in Centromere Satellites From Arabidopsis Relatives*

Posted by on in 2005
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Hall, Sarah E.,*† Song Luo,*‡ Anne E. Hall,*‡ and Daphne Preuss*‡1, 2005, Genetics 170(4): 1913–1927.

*Howard Hughes Medical Institute, University of Chicago, Chicago, Illinois 60637
†Committee on Genetics, University of Chicago, Chicago, Illinois 60637
‡Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637

Abstract

Higher eukaryotic centromeres contain thousands of satellite repeats organized into tandem arrays. As species diverge, new satellite variants are homogenized within and between chromosomes, yet the processes by which particular sequences are dispersed are poorly understood. Here, we isolated and analyzed centromere satellites in plants separated from Arabidopsis thaliana by 5–20 million years, uncovering more rapid satellite divergence compared to primate α-satellite repeats. We also found that satellites derived from the same genomic locus were more similar to each other than satellites derived from disparate genomic regions, indicating that new sequence alterations were homogenized more efficiently at a local, rather than global, level. Nonetheless, the presence of higher-order satellite arrays, similar to those identified in human centromeres, indicated limits to local homogenization and suggested that sequence polymorphisms may play important functional roles. In two species, we defined more extensive polymorphisms, identifying physically separated and highly distinct satellite types. Taken together, these data show that there is a balance between plant satellite homogenization and the persistence of satellite variants. This balance could ultimately generate sufficient sequence divergence to cause mating incompatibilities between plant species, while maintaining adequate conservation within a species for centromere activity.

Note: The Anti-IgY agarose used in this publication was manufactured by Gallus Immunotech.

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