ProcedureChickens were immunized with a B7-H3-Ig representing the extracellular domains of human B7-H3 fused to the hinge region and the CH2 and CH3 domains of human IgG1 (described in Leitner et al., EJI, 2009 39(7) 1754-64). After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared.
SpecificationsThis antibody is suitable for immuofluorescent techniques using the antibody at between 5 and 20 ug/ml (see image). Optimal working dilutions should be determined for your particular assay conditions.
NoteFor in vitro diagnostic‚ research or proteomics applications only.