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Chicken anti-Hexahistidine (HH)

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Cat No.:
AHH
Antibody:
Full NameAffinity Purified Chicken Anti-HexaHistidine (6His)
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeAffinity purified
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.1% Sodium Azide
ProcedureChickens were immunized with HHHHHH conjugated to KLH. After multiple immunization, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Specifications ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction. Appropriate specificity controls were run. Western Blots: 293T cells were transfected with three different constructs of differing sizes. Whole cell extracts were analyzed and the results compared with lysates from non- transfected cells. The anti-HHHHHH was an efffective antibody at a 1:1000 (1 ug/ml) dilution. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding with untransfected extracts. Also effective with Invitrogen Positope.
Storage4°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$165.00
Cat No.:
AHH-F
Antibody:
Full NameFITC Conjugated Affinity Purified Chicken Anti-HexaHistidine (6His)
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeFluorescein conjugated
Physical StateLiquid
Concentration0.5 mg/mL
DiluentPhosphate Buffered Saline‚ pH 7.2 with 0.1% BSA‚ 50% Glycerol
Preservative0.05% sodium azide
ProcedureChickens were immunized with HHHHHH conjugated to KLH. After multiple immunization, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and conjugated to fluorescein isothiocyanate.
SpecificationsPrior to conjugation, the antibodies were analyzed by ELISA and Western Blot analyses. The molar F/P ratio is 3-6. Western Blots: Recommended dilution range is 1:1000 - 1: 5000.
Storage-20°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$185.00
Cat No.:
AHH-HRP
Antibody:
Full NameHRP-Conjugated Affinity Purified Chicken Anti-HexaHistidine (6His)
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeHRP conjugated
Physical StateLiquid
Concentration0.5 mg/mL
Diluent25 mM HEPES pH 7.0‚ 0.05 M NaCl‚ 0.1% BSA‚ 50% glycerol
Preservative0.005% Thimerosal
ProcedureChickens were immunized with HHHHHH conjugated to KLH. After multiple immunization, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and conjugated to horseradish peroxidase (HRP).
SpecificationsELISA: HRP conjugated Chicken anti-6HIS diluted 1:5,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. Western Blots: The HRP conjugated Chicken anti-6HIS was an effective probe at a 1:500 dilution.
Storage-20°C
Price:
$200.00

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