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Chicken anti-Hemagglutinin (HA)

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Cat No.:
AHA
Antibody:
Full NameAffinity Purified Chicken Anti HA (Hemagglutinin) Epitope
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeAffinity purified
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.1% Sodium Azide
ProcedureChickens were immunized with YPYDVPDYA (influenza hemagglutinin-HA- epitope) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
SpecificationsELISA: The anti HA diluted 1:10,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. In Western Blot analyses, the chicken anti-HA was an effective probe at 1:500 dilution. This antibody has also been verified in flow cytometry applications (see embedded figure).
Storage4°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$165.00
Cat No.:
AHA-F
Antibody:
Full NameFITC-Conjugated Affinity Purified Chicken Anti HA (Hemagglutinin) Epitope
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeFluorescein conjugated
Physical StateLiquid
Concentration0.5 mg/mL
DiluentPhosphate Buffered Saline‚ pH 7.2 with 0.1% BSA‚ 50% Glycerol
Preservative0.05% sodium azide
Procedure Chickens were immunized with YPYDVPDYA (influenza hemagglutinin-HA- epitope) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and the antibodies were conjugated to fluorescein isothiocyanate (FITC).
SpecificationsPrior to conjugation the affinity purified antibodies were analyzed using ELISA and Western Blot analyses. Molar F/P ratio is 3.6.
Storage-20°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$185.00
Cat No.:
AHA-HRP
Antibody:
Full Name HRP-Conjugated Affinity Purified Chicken Anti HA (Hemagglutinin) Epitope
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeHRP conjugated
Physical StateLiquid
Concentration0.5 mg/mL
Diluent25 mM HEPES pH 7.0‚ 0.05 M NaCl‚ 0.1% BSA‚ 50% glycerol
Preservative0.005% Thimerosal
ProcedureChickens were immunized with YPYDVPDYA (influenza hemagglutinin-HA- epitope) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and the antibodies were conjugated to horseradish peroxidase (HRP).
SpecificationsELISA: The anti HA diluted 1:5,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. In Western Blot analyses, the chicken anti-HA was an effective probe at 1:500 dilution.
Storage-20°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$200.00

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