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Chicken anti-Green Fluorescent Protein (GFP)

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AGFP
Antibody:
Full NameChicken anti-Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae]
HostChicken
Quantity10 mg
Volume0.5 mL
TypeEgg yolk-derived purified IgY
Physical StateLiquid
Concentration20.0 mg/mL
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.075% sodium azide
ProcedureChickens were immunized with recombinant full-length bacterial gonococci Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae] expressed in E. coli cells. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified.
SpecificationsIn a Western blot, the anti-GFP used at between 1:3000 and 1:5000 dilution could detect 10 ng of GFP in an A549 cell lysate (see image). Optimal conjugate working dilutions should be determined for your particular assay conditions.
Storage4°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$220.00

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