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Chicken anti-FLAG (DYDDDDK)

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Cat No.:
AFLAG
Antibody:
Full NameAffinity Purified Chicken Anti FLAG Epitope (DYKDDDDK)
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeAffinity purified
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.1% Sodium Azide
ProcedureChickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunizations, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
SpecificationsAntibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40. ELISA: We suggest a 1:5,000-1:10,000 dilution. WESTERN BLOTS: We suggest a 1:2,000 to 1:10,000 dilution. For immunohistochemistry, we suggest a 1:500 dilution (see image). Please note that the investigator must determine the appropriate dilution for your particular application.
Storage4°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$165.00
Cat No.:
AFLAG-F
Antibody:
Full NameFITC-conjugated Affinity Purified Chicken Anti FLAG Epitope (DYKDDDDK)
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeFluorescein conjugated
Physical StateLiquid
Concentration0.5 mg/mL
Diluent25 mM HEPES pH 7.0‚ 0.05 M NaCl‚ 0.1% BSA‚ 50% glycerol
Preservative0.05% sodium azide
ProcedureChickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunizations, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and conjugated with fluorescein isothiocyanate (FITC).
SpecificationsPrior to conjugation, the antibodies were analyzed by ELISA and Western Blot. The molar F/P ratio is 4.1. Please note that the investigator must determine the appropriate dilution for your particular application. ELISA: We suggest a 1:2500-1:10,000 dilution. WESTERN BLOTS: We suggest a 1:500 to 1:2500 dilution.
Storage-20°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$180.00
Cat No.:
AFLAG-HRP
Antibody:
Full NameHorseradish peroxidase conjugated Affinity Purified Chicken Anti FLAG Epitope
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeHRP conjugated
Physical StateLiquid
Concentration0.5 mg/mL
Diluent25 mM HEPES pH 7.0‚ 0.05 M NaCl‚ 0.1% BSA‚ 50% glycerol
Preservative0.005% Thimerosal
ProcedureChickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunization, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and then conjugated to HRP.
SpecificationsELISA: We suggest a 1:2500-1:10,000 dilution. WESTERN BLOTS: We suggest a 1:500 to 1:2500 dilution. Please note that the investigator must determine the appropriate dilution for your particular application.
Storage-20°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$200.00

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