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Chicken anti-CMYC (EQKLISEEDL)

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Cat No.:
ACMYC
Antibody:
Full NameAffinity Purified Chicken Anti C-MYC Epitope Tag
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeAffinity purified
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.1% Sodium Azide
ProcedureChickens were immunized with EQKLISEEDL (C-MYC) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
SpecificationsELISA: coating: 1:50 – 1:200; probing: 1:5000 - 1:20,000 Western Blots: 1:5000 dilution. Flow Cytometry: 1:100 - 1:300 dilution (see embedded figures).
Storage4°C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$165.00
Cat No.:
ACMYC-B
Antibody:
Full Name Biotin-Conjugated Affinity Purified Chicken Anti C-MYC Epitope Tag
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeBiotin conjugated
Physical StateLiquid
Concentration0.5 mg/mL
Diluent25 mM HEPES pH 7.0‚ 0.05 M NaCl‚ 0.1% BSA‚ 50% glycerol
Preservative0.005% Thimerosal
ProcedureChickens were immunized with EQKLISEEDL (C-MYC) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and conjugated biotin.
SpecificationsFor ELISA, a 1:2500 - 1:10,000 dilution is recommended Western Blots: The anti C-MYC was an effective probe at 1:500 - 1:1500 dilution.
Storage-20°C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$185.00
Cat No.:
ACMYC-F
Antibody:
Full NameFITC- Conjugated Affinity Purified Chicken Anti C-MYC Epitope Tag
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeFluorescein conjugated
Physical StateLiquid
Concentration0.5 mg/mL
DiluentPhosphate Buffered Saline‚ pH 7.2 with 0.1% BSA‚ 50% Glycerol
Preservative0.05% sodium azide
ProcedureChickens were immunized with EQKLISEEDL (C-MYC) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. The antibodies were conjugated with fluorescein isothiocyanate (FITC).
Specifications Prior to conjugation, the antibodies were analyzed by Western blot. We recommend a 1:500 dilution for Western blot analysis. The F/P ration is between 3 and 6.
Storage-20°C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$195.00
Cat No.:
ACMYC-HRP
Antibody:
Full NameHRP-Conjugated Affinity Purified Chicken Anti C-MYC Epitope Tag
HostChicken
Quantity0.1 mg
Volume0.2 mL
TypeHRP conjugated
Physical StateLiquid
Concentration0.5 mg/mL
Diluent25 mM HEPES pH 7.0‚ 0.05 M NaCl‚ 0.1% BSA‚ 50% glycerol
Preservative0.005% Thimerosal
ProcedureChickens were immunized with EQKLISEEDL (C-MYC) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and conjugated to horseradish peroxidase.
SpecificationsAntibody specificity was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL-BSA). A 1:10,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction. Appropriate specificity controls were run. Western Blots: The anti C-MYC was an effective probe at 1:1000 dilution.
Storage-20°C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$200.00

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