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Given previous evidence for CART (cocaine- and amphetamine-regulated transcript) signaling in AtT20 cells, the binding of [125I]-CART61-102 was characterized in these cells. The binding was specific, saturable, dependent on time, pH, temperature and protein concentration, with a Bmax of 101.4+/-8.8 fmol/mg protein and a Kd of 21.9+/-8.0 pM. Only active CART55-102, but not other peptides or drugs, inhibited the [125I]-CART61-102 binding. These data are the first demonstration of specific receptor binding for CART peptides.
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