Full NameAffinity Purified Chicken Anti FLAG Epitope (DYKDDDDK)
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.1% Sodium Azide
ProcedureChickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunizations, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
SpecificationsAntibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40. ELISA: We suggest a 1:5,000-1:10,000 dilution. WESTERN BLOTS: We suggest a 1:2,000 to 1:10,000 dilution. For immunohistochemistry, we suggest a 1:500 dilution (see image). Please note that the investigator must determine the appropriate dilution for your particular application.
NoteFor in vitro diagnostic‚ research or proteomics applications only.