Full NameAffinity-purified Chicken-anti-Rat IgG (H+L)
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.1% Sodium Azide
ProcedureChickens were immunized with highly purified rat IgG isolated from normal pooled rat serum. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent.
SpecificationsThe affinity purified anti rat IgG reacts with immunoglobulins and the papain derived Fc and Fab fragments by IEP and gel diffusion techniques.
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Full NameFITC-conjugated Affinity Purified Chicken Anti-Rat IgG (H+L)
DiluentPhosphate Buffered Saline‚ pH 7.2 with 1% BSA‚ 50% Glycerol
Preservative0.05% sodium azide
ProcedureChickens were immunized with highly purified normal rat IgG in Freunds adjuvant. Eggs were collected, the IgY fraction prepared and the anti-rat IgG antibodies immunoaffinity purified from an IgG immunoabsorbant. The antibodies were conjugated with fluorescein isothiocyanate (FITC).
SpecificationsThe affinity purified antibody reacts with rat IgG in whole serum and purified IgG, Fab and Fc fragments when tested by immunoelectrophoresis. Molar F/P ratio = 5.9