Full NameAnti-Chicken IgY Agarose
Quantity1.0 ml bed volume
Volume1 ml settled gel
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.075% sodium azide
Procedure A donkey was immunized with purified chicken IgY. Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
SpecificationsThe specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 ml PBS (0.1 mg/ml) to 1.0 ml bed volume of agarose. Two ml aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per ml agarose.
Storage4°C in the dark. This product has a shelf life of at least 12 months.
NoteFor in vitro diagnostic‚ research or proteomics applications only.