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Anti-Bacillus anthracis antigens

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Cat No.:
ABaLFA
Antibody:
Full NameAffinity Purified Chicken anti-Bacillus anthracis Lethal Factor A
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeAffinity purified
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.075% sodium azide
ProcedureChickens were immunized with highly purified Lethal Factor A purified from Bacillus anthracis. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Bacillus anthracis Lethal Factor A was immunoaffinity purified using the immunogen immobilized on a solid support.
SpecificationsThis antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:10,000 dilution of the IgY was an effective probe for 16 ng of Lethal Factor A. By Direct ELISA, the half-point titre was 1:38,000 dilution using a 2 ug/ml coating of Lethal Factor A. Optimal conjugate working dilutions should be determined for your particular assay conditions.
Storage4°C
NoteFor in vitro diagnostic‚ research or proteomics applications only.
Price:
$220.00
Cat No.:
ABaLFA-B
Antibody:
Full NameBiotinylated Affinity Purified Chicken anti-Bacillus anthracis Lethal Factor A
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeBiotin conjugated
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate Buffered Saline‚ 50% Glycerol
Preservative0.01% Thimerosal
ProcedureChickens were immunized with highly purified Lethal Factor A purified from Bacillus anthracis. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Bacillus anthracis Lethal Factor A was immunoaffinity purified using the immunogen immobilized on a solid support and conjugated to Biotin.
SpecificationsThis biotinylated IgY can be used as a detector antibody (1:1000 - 1:2000 dilution) with the Affinity Purifed Chicken anti-B. anthracis Lethal Factor (Cat. # ABaLFA, 1 ug/ml) as the coating antibody in a sandwich ELISA. Optimal conjugate working dilutions should be determined for your particular assay conditions.
Storage-20° C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$290.00
Cat No.:
ABaLFA-F
Antibody:
Full NameFITC-conjugated Affinity Purified Chicken anti-Bacillus anthracis Lethal Factor A
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeFluorescein conjugated
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate Buffered Saline‚ 50% Glycerol
Preservative0.075% sodium azide
ProcedureChickens were immunized with highly purified Lethal Factor A purified from Bacillus anthracis. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Bacillus anthracis Lethal Factor A was immunoaffinity purified using the immunogen immobilized on a solid support and conjugated to Fluorscein Isothiocyanate.
SpecificationsOptimal conjugate working dilutions should be determined for your particular assay conditions.
Storage-20° C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$310.00
Cat No.:
ABaLFA-HRP
Antibody:
Full NameHRP-Conjugated Affinity Purified Chicken anti-Bacillus anthracis Lethal Factor A
HostChicken
Quantity0.1 mg
Volume0.1 mL
TypeHRP conjugated
Physical StateLiquid
Concentration1.0 mg/mL
DiluentPhosphate Buffered Saline‚ 50% Glycerol
Preservative0.01% Thimerosal
ProcedureChickens were immunized with highly purified Lethal Factor A purified from Bacillus anthracis. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Bacillus anthracis Lethal Factor A was immunoaffinity purified using the immunogen immobilized on a solid support and conjugated to Horseradish Peroxidase.
SpecificationsOptimal conjugate working dilutions should be determined for your particular assay conditions.
Storage-20° C
NoteFor in vitro diagnostic, research or proteomics applications only.
Price:
$330.00

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