Full NameAffinity Purified Chicken anti-Arabidopsis thaliana SEUSS peptide
DiluentPhosphate buffered saline‚ pH 7.2
Preservative0.075% sodium azide
ProcedureChickens were immunized with Arabidopsis thaliana SEUSS peptide (sequence: NQLLAEQQRNKKME) coupled to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. The anti-Arabidopsis thaliana SEUSS antibody was immunoaffinity purified using the peptide mmobilized on a solid support.
SpecificationsThis antibody has been successfully used to detect the Arabidopsis thaliana SEUSS protein in Western blots. Recommended dilutions for Western blotting is 1:40,000, incubated at 4°C overnight, used with HRP-Donkey anti-IgY at 1:20,000 (1 hour incubation at room temperature). However, optimal working dilutions for this antibody should be determined for your particular assay conditions.
NoteFor in vitro diagnostic‚ research or proteomics applications only.