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Adjuvant-dependent immunogenicity of Staphylococcus aureus Efb and Map proteins in chickens.

Posted by on in 2015
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Grzywa R1Walczak M1Łupicka-Słowik A1Bobrek K2Boivin S3Brown EL4Gaweł A2Stefaniak T5Oleksyszyn J1Sieńczyk M6. 2015. Vet Immunol Immunopathol. 166(1-2):50-6. doi: 10.1016/j.vetimm.2015.04.009. Epub 2015 May 16.

1Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
2Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Epizootiology and Clinic of Bird and Exotic Animals, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland.
3European Molecular Biology Laboratory (EMBL), Notkestraße 85 c/o DESY, Building 25A, 22603 Hamburg, Germany.
4Center for Infectious Diseases, University of Texas School of Public Health, Houston, TX 77030, USA.
5Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Norwida 31, 50-375 Wroclaw, Poland.
6Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland. Electronic address: marcin.sienczyk@pwr.edu.pl.

Abstract

The avian IgY antibodies generated in hens and isolated from egg yolk have gained in popularity as they present an alternative source of antibodies for diagnostic as well as therapeutic applications. One of the advantages of IgY technology are the large amounts of produced antibodies from a single animal combined with their high reactivity representing an attractive alternative for mammalian antibodies. Despite many known protocols for the immunization of chickens, the administration of new antigens often requires additional modification such as antigen dose or use of an adjuvant in order to elicit a significant immune response. We investigated the immunogenicity of three Staphylococcus aureus antigens including two extracellular proteins Map and Efb and one selected Efb105-124 epitope conjugated to KLH that were administered to the animals. Additionally, the immunization protocol included two adjuvant systems: Freund's complete adjuvant and Emulsigen-D. The results demonstrated a high immunostimulatory potency of Freund's complete adjuvant, especially in case of Efb compared to the immune response elicited by Emulsigen-D. However, after immunization with the KLH-Efb105-124 conjugate, the obtained antibodies showed similar reactivity regardless of adjuvant system used with the only exception being their avidity.

Copyright © 2015 Elsevier B.V. All rights reserved.

KEYWORDS:

Adjuvant; Extracellular fibrinogen binding protein; Immunoglobulin Y; MHC class II analog protein; Staphylococcus aureus

PMID:
 
26004944
 
[PubMed - in process]
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