Standing out in the field of IgY Immunotechnology

Comparison of IgG, IgE, IgY and IgY (ΔFc)

 
IgG
IgE
IgY
IgY (ΔFc)
Species

Mammals

Mammals

Birds, Reptiles, Amphibians Lungfish

Anseriform Birds, Amphibians, Lungfish

Molecular Weight (kD)

150

200

180

118

Isoelectric
Point (pI)

6.4-9.0  (Ref. 1)

5.2-5.8  (Ref. 2)

5.7-7.6  (Ref. 3)

5.2-7.3 (Ref. 4)

Extinction
Coefficient*

1.40

1.62  (Ref. 5)

1.36

1.38

No. Constant Domains

4

4

3

2

Carbohydrate
Content (%)

2-4

12

(Ref. 6)

0.6  (Ref. 7)

Hinge Region

Yes

No

No

No

Antigen Valency

2

2

2

2

Major Serum
Antibody

Yes

No

Yes

Yes

Source

Serum

Serum

Serum/EggYolk

Serum/Egg Yolk

 Concentration
(mg/ml)

10-12 

10-4

Chicken:

Serum: 8-10 (Ref. 10)

Yolk: 10-20  (Ref. 9)

Duck:

Yolk: IgY (ΔFc): 3-12

(Ref. 4)

Mammalian Complement binding

Yes

No

No

No

Rheumatoid Factor Binding

Yes

No

No

No

Fc receptor binding

Yes

No

No

No

Mediates
Anaphylaxis

No

Yes

Yes

No

Binding to Protein A

Yes

No

No

No

Binding to Protein G

Yes

No

No

No

Binding to Protein L

Yes

Yes

No  (Ref. 8)

No

* optical density of a 1.0 mg/ml solution at 280 nm.

Other "About IgY" topics:

References:
1. Li G, Stewart R, Conlan B, Gilbert A, Roerth, P, Nair H. (2002) Purification of human immunoglobulin G: a new approach to plasma fractionation. Vox Sanguinis 83 : 332-338.
2. Clerc, M. -F., Granato, D. A.  and M. Horisberger, (1988). Labelling of colloidal gold with IgE. Histochemistry and Cell Biology 89 : 343-349.
3. Dávalos-Pantoja L, Ortega-Vinuesa JL, Bastos-González D, Hidalgo-Alvarez R, (2000)  A comparative study between the adsorption of IgY and IgG on latex particles.  J Biomater Sci Polym Ed. 11:657-73. 
4. Chiou, Victor, 2003. Process fo selectively isolating IgY antibodies from egg yolk of an anseriform bird and IgY antibodies obtained thereby. United States Patent Application Publication 20060223986.
5. Liu, F.T., Bohn, J.W., Ferry, E.L., Yamamoto, H., Molinaro, C.A., Sherman, L.A., Klinman, N.R., and Katz, D.H. (1980) Monoclonal dinitrophenyl-specific murine IgE antibody preparation, isolation and characterization. J. Immunol. 124 : 2728-2736.
6. Ohta M, Hamako J, Yamamoto S, Hatta H, Kim M, Yamamoto T, Oka S, Mizuochi T, Matsuura F., 1991, Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein.
7. Zimmerman, B., Shalatin, N. and H.M. Grey (1971).  Structural studies on the duck 5.7S and 7.8S immunoglobulins. Biochemistry 10 : 482-488.
8. Nilsson, E. Larsson, A.  (2005) Chicken Anti-Protein L for the Detection of Small Amounts of Protein L in the Presence of IgG.  Hybridoma. 24(2): 112-114
9. Carlander, D. 2002.Avian IgY Antibody. In vitro and in vivo. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1119.53 pp. Uppsala. ISBN 91-554-5227-2
10. Wang, Y.W., Cherian, G., Sunwoo, H.H and Sim. J.S. 2000. Dietary polyunsaturated fatty acids significantly affect laying hen lymphocyte proliferation and immunoglobulin G concentration in serum and egg yolk. Can. J. Anim. Sci. 80:597-604.